Histone H2A antibody (N-Term)

Catalog No. ABIN127253

The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and IP.

Quick Overview for Histone H2A antibody (N-Term) (ABIN127253)

Target: Histone H2A

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Histone H2A Antibody

Binding Specificity: N-Term

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to the N-terminus of human histone H2A

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Histone H2A

Target: Histone H2A

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H2A Histone Family, HIST1H2A