Histone 3 antibody (C-Term)

Catalog No. ABIN127255

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone 3 antibody (C-Term) (ABIN127255)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone 3 Antibody

Binding Specificity: C-Term

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide near the C-terminus of human Histone H3

Application Details

Application Notes: Western blot: 0.5-4 μg/mL. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H3 Histone family, HIST1H3