beta Actin antibody

Catalog No. ABIN127292

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB. It is suitable to detect beta Actin in samples from Human, Mouse and Rabbit. There is 1 publication available.

Quick Overview for beta Actin antibody (ABIN127292)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-beta Actin Antibody

Specificity: The antibody recognizes 45 kDa beta-Actin.

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide corresponding to residues around amino acid 10 of Human Actin.

Application Details

Application Notes: Western Blot: 0.5-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.01 % Thimerosal, 0.5 % BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

References for anti-beta Actin antibody (ABIN127292)

Ishizuka, Hinata, Watanabe: "Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells." in: The Journal of endocrinology, Vol. 208, Issue 2, pp. 147-59, (2011) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin beta / ACTB

Background: Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction