GST antibody

Catalog No. ABIN129505

Supplier Product No.: 200-301-200

This Mouse Monoclonal antibody specifically detects GST in WB, ELISA, IP and IHC. It exhibits reactivity toward Schistosoma japonicum.

Quick Overview for GST antibody (ABIN129505)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: 3D4

Product Details anti-GST Antibody

Supplier Product No.: 200-301-200

Supplier: Rockland

Purpose: GST Antibody

Cross-Reactivity (Details): Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Characteristics: Synonyms: mouse anti-GST antibody, Glutathione-S-Transferase, Anti-GST monoclonal antibody

Purification: Anti-GST monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography which reacts specifically with GST.

Sterility: Sterile filtered

Immunogen:

Immunogen: A BALB/c mouse was immunized with Glutathione-S-Transferase [Schistosoma japonicum].

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GST antibody has been tested by ELISA and western blot and is suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:50,000 - 1:150,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store anti-GST at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST

Background: Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.