BUB1 antibody (AA 281-419)

Catalog No. ABIN129514

Supplier Product No.: 200-301-900

This Mouse Monoclonal antibody specifically detects BUB1 in WB, ELISA, IF, IP and FM. It exhibits reactivity toward Human.

Quick Overview for BUB1 antibody (AA 281-419) (ABIN129514)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: 14H5

Product Details anti-BUB1 Antibody

Binding Specificity: AA 281-419

Supplier Product No.: 200-301-900

Supplier: Rockland

Purpose: BUB1 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against human BUB1 protein.

Characteristics: Synonyms: mouse anti-BUB1 antibody, Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody

Purification: The product was purified from tissue culture supernatant by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8 OR 100 % methanol at -20° C.  Permeabilize fixed cells with 0.5 % Triton X-100.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1

Background: Background: BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint.  Mutations in this gene have been associated with aneuploidy and several forms of cancer.   BUB1 is autophosphorylated when cells enter mitosis.  This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine.  Expression is associated with cells/tissues with a high mitotic index.

Gene ID: 699

NCBI Accession: NP_004327

UniProt: O43683