RNF34 antibody (AA 1-373)

Catalog No. ABIN129536

Supplier Product No.: 200-401-933

Product Details anti-RNF34 Antibody

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Binding Specificity: AA 1-373

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 200-401-933

Supplier: Rockland

Purpose: RNF34 Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against human RNF34 protein.

Characteristics: Synonyms: rabbit anti-RNF34 Antibody, rabbit anti-RNF 34 Antibody, Caspase regulator CARP1 antibody, CARP 1 antibody, hRFI antibody, RFI antibody, RIFF antibody, Ring finger protein 34 antibody

Purification: The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:4,000 - 1:20,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RNF34 (RNF34 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation.  RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.

Gene ID: 80196, 37595539

UniProt: Q969K3