RNF2 antibody (AA 180-205)

Catalog No. ABIN129557

Supplier Product No.: 600-101-292

The Goat Polyclonal anti-RNF2 antibody has been validated for WB, ELISA, IF, FM and MA. It is suitable to detect RNF2 in samples from Human, Mouse and Chinese Hamster.

Quick Overview for RNF2 antibody (AA 180-205) (ABIN129557)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human, Mouse, Chinese Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Product Details anti-RNF2 Antibody

Binding Specificity: AA 180-205

Supplier Product No.: 600-101-292

Supplier: Rockland

Purpose: RING1B Antibody

Cross-Reactivity (Details): Affinity purified Anti-RING1B antibody is directed against human RING1B protein.

Characteristics: Synonyms: goat anti-RING1B Antibody, RING finger protein BAP1 antibody, DING antibody, RING finger protein 1B, E3 ubiquitin protein ligase RING 2 antibody, Huntingtin interacting protein 2 interacting protein 3 antibody, Anti-RNF2 antibody, HIP2-interacting protein 3, Protein DinG

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-RING1B purified antibody has been tested for use in ELISA, immunofluorescence, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:25,000

IF Microscopy Dilution: 1:300

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: RNF2

Background: Background: RING1B (also known as BAP1 and RNF2) is one of the PcG proteins.  The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity. Anti-RINGB1 Antibody is useful for researchers interested in epigenetics, ubiquitin, and transcription factor research.

Gene ID: 6045, 6005747

UniProt: Q99496