NCS1 antibody

Catalog No. ABIN129571

Supplier Product No.: 600-401-216

This Rabbit Polyclonal antibody specifically detects NCS1 in WB, ELISA, IF, MA and FM. It exhibits reactivity toward Mouse.

Quick Overview for NCS1 antibody (ABIN129571)

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Product Details anti-NCS1 Antibody

Supplier Product No.: 600-401-216

Supplier: Rockland

Purpose: Frequenin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-FREQUENIN antibody, NCS-1, FLUP, FREQ, Neuronal calcium sensor 1, frequenin homolog antibody, Frequenin-like protein antibody, NCS 1 antibody, NCS1 antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:100-1:2500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: Ncs1

Background: Background: Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Gene ID: 14299

NCBI Accession: NP_062655

UniProt: Q8BNY6