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PLK1 antibody (Polo-Like Kinase 1) (AA 205-214) Primary Antibody

PLK1 Reactivity: Human IHC, ELISA, WB Host: Rabbit Polyclonal
Pubmed (1)
Catalog No. ABIN129581
$613.50
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100 μg
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  • Target
    Binding Specificity
    AA 205-214, pThr210
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This PLK1 antibody is un-conjugated
    Application
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
    Immunogen
    Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.
    Isotype
    IgG
  • Application Notes
    This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract.   This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, 2009 (PubMed).

  • Target
    Alternative Name
    Plk1
    Background
    Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1.  Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression.  Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.  Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
    Synonyms: PLK 1 antibody, PLK antibody, polio like kinase antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody
    Gene ID
    5347, 21359873
    UniProt
    P53350
    Pathways
    Cell Division Cycle, M Phase
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