PLK1 antibody (Polo-Like Kinase 1) (AA 331-508) Primary Antibody
PLK1 Reactivity: Human ICC, ELISA, WB Host: Mouse Monoclonal 1D1
Catalog No. ABIN969580
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- Binding Specificity
- AA 331-508
- This PLK1 antibody is un-conjugated
- Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
- Purified recombinant fragment of human PLK1 (AA 331-508) expressed in E. Coli.
- Application Notes
- ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:50
- For Research Use only
- PBS with 0.05 % sodium azide
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C,-20 °C
- Storage Comment
- 4°C, -20°C for long term storage
Lee, Rhee: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, 2011 (PubMed).
Li, Zhou, Ye, Cheng, Zhou, Lu, Xie: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, 2011 (PubMed).
- Lee, Rhee: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, 2011 (PubMed).
- Alternative Name
- PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation.
- Molecular Weight
- 68 kDa
- Gene ID
- Cell Division Cycle, M Phase
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