LIM Domain Binding 2 Protein antibody (AA 100-125)

Catalog No. ABIN129586

Supplier Product No.: 600-401-459

This Rabbit Polyclonal antibody specifically detects LIM Domain Binding 2 Protein in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for LIM Domain Binding 2 Protein antibody (AA 100-125) (ABIN129586)

Target: LIM Domain Binding 2 Protein (LDB2) (LIM Domain Binding 2 (LDB2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 2 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LIM Domain Binding 2 Protein Antibody

Binding Specificity: AA 100-125

Supplier Product No.: 600-401-459

Supplier: Rockland

Purpose: Ldb2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse LDB2.

Characteristics: Synonyms: rabbit anti-LDB2 antibody, rabbit anti-LDB2/CLIM1 antibody, rabbit anti-CLIM1 antibody, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LIM Domain Binding 2 Protein

Target: LIM Domain Binding 2 Protein (LDB2) (LIM Domain Binding 2 (LDB2))

Alternative Name: Ldb2

Background: Background: LDB2 (LIM homeobox protein cofactor CLIM-1a).  It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1.  LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95 % , 73 % and 62 % amino acid identity with mouse Ldb1, respectively.

Gene ID: 16826, 116642898

UniProt: O55203

Pathways: Stem Cell Maintenance