FBXO43 antibody (N-Term)

Catalog No. ABIN129598

Supplier Product No.: 600-401-485

This Rabbit Polyclonal antibody specifically detects FBXO43 in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse, Rat and Chimpanzee.

Quick Overview for FBXO43 antibody (N-Term) (ABIN129598)

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO43 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-FBXO43 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-485

Supplier: Rockland

Purpose: Fbp5B Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Fbp5B protein.

Characteristics: Synonyms: rabbit anti-F-Box Protein 43/Fbp5B antibody, rabbit anti-F-Box Protein 43 antibody, rabbit anti-Fbp5B antibody, F-box only protein 43, Endogenous meiotic inhibitor 2, FBXO43, EMI2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 kDa in size corresponding to Fbp5B protein by western blotting in the appropriate cell lysate or extract.  Predicted molecular weight is 51 kDa.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FBXO43

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Alternative Name: FBXO43

Background: Background: Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.

Gene ID: 286151, 117606351

UniProt: Q4G163