ATG7 antibody (AA 550-575)

Catalog No. ABIN129600

Supplier Product No.: 600-401-487

This anti-ATG7 antibody is a Rabbit Polyclonal antibody detecting ATG7 in WB, ELISA and FM. Suitable for Human. This Primary Antibody has been cited in 7+ publications.

Quick Overview for ATG7 antibody (AA 550-575) (ABIN129600)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Product Details anti-ATG7 Antibody

Binding Specificity: AA 550-575

Supplier Product No.: 600-401-487

Supplier: Rockland

Purpose: APG7 Antibody

Cross-Reactivity (Details): Reactivity occurs against human Apg7 protein.

Characteristics: Synonyms: rabbit anti-APG7 Antibody, APG-7, APG 7, APG7 autophagy 7-like antibody, APG7 like antibody, APG7L antibody, ATG 7 antibody, ATG-7 antibody, ATG7 autophagy related 7 homolog antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550-575 of Human Apg7 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract.   Human Apg7 has been reported to form a homodimer at approximately 160 kDa.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:300,000 - 1:400,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.46 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ATG7 antibody (ABIN129600)

Bhattacharya, Parillon, Zeng, Han, Eissa: "Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis." in: The Journal of biological chemistry, Vol. 289, Issue 38, pp. 26525-32, (2015) (PubMed).

Abdel Fattah, Bhattacharya, Herron, Safdar, Eissa: "Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 11, pp. 5407-16, (2015) (PubMed).

Fung, Chen, Grandis, Duvvuri: "EGFR tyrosine kinase inhibition induces autophagy in cancer cells." in: Cancer biology & therapy, Vol. 13, Issue 14, pp. 1417-24, (2013) (PubMed).

Shin, Fattah, Bhattacharya, Ko, Eissa: "Inflammasome activation by altered proteostasis." in: The Journal of biological chemistry, Vol. 288, Issue 50, pp. 35886-95, (2013) (PubMed).

Liu, Ko, Xu, Fattah, Xiang, Jagannath, Ishii, Komatsu, Eissa: "Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress." in: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19687-98, (2012) (PubMed).

Sarkar, Perlstein, Imarisio, Pineau, Cordenier, Maglathlin, Webster, Lewis, OKane, Schreiber, Rubinsztein: "Small molecules enhance autophagy and reduce toxicity in Huntington's disease models." in: Nature chemical biology, Vol. 3, Issue 6, pp. 331-8, (2007) (PubMed).

Sarkar, Davies, Huang, Tunnacliffe, Rubinsztein: "Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein." in: The Journal of biological chemistry, Vol. 282, Issue 8, pp. 5641-52, (2007) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background: Background: Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

Gene ID: 10533

NCBI Accession: NP_001129503

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy