SLIT2 antibody (AA 475-500)

Catalog No. ABIN129628

Supplier Product No.: 600-401-846

This Rabbit Polyclonal antibody specifically detects SLIT2 in WB, IHC and ELISA. It exhibits reactivity toward Human and Chicken.

Quick Overview for SLIT2 antibody (AA 475-500) (ABIN129628)

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Reactivity: Human, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SLIT2 Antibody

Binding Specificity: AA 475-500

Supplier Product No.: 600-401-846

Supplier: Rockland

Purpose: SLIT2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SLIT-2 protein.

Characteristics: Synonyms: rabbit anti-SLIT-2 antibody, SLIT2, SLIT 2, Slit homolog 2 protein, SLIL3, SLIL-3 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  For western blotting block the membrane with goat serum.  Do not block with non-fat dry milk.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SLIT2

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Alternative Name: SLIT2

Background: Background: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors.  SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play a role in guiding commissural axons once they reach the floor plate by modulating the response to netrin.  In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex.  In vitro, only commissural axons that crossed the midline responded to SLIT-2.  In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm.  SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord.  Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined.  Multiple isoforms have been reported for this product.

Gene ID: 9353, 4759146

UniProt: O94813

Pathways: Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration