GGA1 antibody (AA 330-360)

Catalog No. ABIN129633

Supplier Product No.: 600-401-853

Product Details anti-GGA1 Antibody

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Binding Specificity: AA 330-360

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-853

Supplier: Rockland

Purpose: GGA1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human GGA1 protein.

Characteristics: Synonyms: rabbit anti-GGA-1 rabbit, GGA 1,GGA1, ADP-ribosylation factor binding protein, Golgi-localized gamma ear-containing ARF-binding protein 1, Gamma-adaptin related protein 1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at 70 kDa in size corresponding to GGA1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.35 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GGA1

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Alternative Name: GGA1 (GGA1 Products)

Background: Background: GGA1 (also known as ADP-ribosylation factor binding protein, Golgi-localized, gamma ear-containing, ARF-binding protein 1, Gamma-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID: 26088, 9558729

UniProt: Q9UJY5