Swi6 (AA 314-328) antibody

Catalog No. ABIN129643

Supplier Product No.: 600-401-867

This Rabbit Polyclonal antibody specifically detects Swi6 in WB and ELISA. It exhibits reactivity toward Schizosaccharomyces pombe.

Quick Overview for Swi6 (AA 314-328) antibody (ABIN129643)

Target: Swi6

Reactivity: Schizosaccharomyces pombe

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 314-328

Supplier Product No.: 600-401-867

Supplier: Rockland

Purpose: Swi6 Antibody

Cross-Reactivity (Details): BLAST analysis indicates that cross reactivity with homologues of this protein from other sources is not likely.

Characteristics: Synonyms: rabbit anti-Swi6 antibody, Chromatin-associated protein swi6, swi-6, swi 6, SPAC664.01c, SPAC824.10c

Purification: Swi6 Antibody is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: This anti-Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:30,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Swi6 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Swi6

Background: Background: Swi6 is also known as chromatin-associated swi6 protein and repression of silent mating type loci protein.  This protein recognizes and binds to histone H3 tails that are methylated at Lys-9, leading to epigenetic repression. Swi6 is also involved in the repression of the silent mating-type loci MAT2 and MAT3 and it may be responsible for the compaction of MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 also interacts with the cohesion subunit psc3. Swi6 has a nuclear localization and contains 2 chromo domains.

Gene ID: 2541633, 19114361

UniProt: P40381