CAMK2A antibody (N-Term)

Catalog No. ABIN129648

Supplier Product No.: 600-401-873

This anti-CAMK2A antibody is a Rabbit Polyclonal antibody detecting CAMK2A in WB and ELISA. Suitable for Mouse.

Quick Overview for CAMK2A antibody (N-Term) (ABIN129648)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CAMK2A Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-873

Supplier: Rockland

Purpose: CaM Kinase II Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human CaM Kinase II protein.

Characteristics: Synonyms: rabbit anti-CaM Kinase II Antibody, Calcium/calmodulin dependent protein kinase II alpha antibody, CaM kinase II alpha antibody, CaM kinase II α chain antibody, CaM kinase II alpha subunit antibody, CAMK2A, CAMKA

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human CaM Kinase II protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 54 kDa in size corresponding to CaM Kinase II by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:3,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.57 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A

Background: Background: CaM Kinase II (also known as CAMK2 and calcium/calmodulin-dependent protein kinase type II alpha chain) is a prominent kinase in the central nervous system that may function in the long-term potentiation of neurotransmitter release.  CaM Kinase II autophosphorylates itself at Thr-286 which allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CaM Kinase II is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. This kinase is expressed in brain tissue.  Alternative splicing occurs for this gene product.

Gene ID: 815

NCBI Accession: NP_741960

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction