CDC16 antibody (pThr580)

Catalog No. ABIN129649

Supplier Product No.: 600-401-876

This Rabbit Polyclonal antibody specifically detects CDC16 in WB, ELISA and FM. It exhibits reactivity toward Human.

Quick Overview for CDC16 antibody (pThr580) (ABIN129649)

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Product Details anti-CDC16 Antibody

Binding Specificity: pThr580

Supplier Product No.: 600-401-876

Supplier: Rockland

Purpose: APC6 phospho T580 Antibody

Cross-Reactivity (Details): This antibody is specific for phosphorylated human APC6 protein at the pT580 residue.

Characteristics: Synonyms: rabbit anti-APC6 pT580 Antibody, APC 6, APC-6, Anaphase promoting complex subunit 6 antibody, CDC16Hs antibody, Cell division cycle 16 homolog antibody, Cell division cycle protein 16 homolog antibody, Cyclosome subunit 6 antibody

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:1,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.97 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CDC16

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Alternative Name: CDC16

Background: Background: APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle.   The APC/C is composed of at least 11 subunits.   APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.  Splice variants have been described for this protein.

Gene ID: 8881, 37537763

UniProt: Q13042

Pathways: Regulation of Actin Filament Polymerization