CD74 antibody

Catalog No. ABIN192403

This Mouse Monoclonal antibody specifically detects CD74 in FACS, WB, IP, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD74 antibody (ABIN192403)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LN2

Product Details anti-CD74 Antibody

Purpose: Anti-Hu CD74 Purified

Specificity: The antibody LN2 recognizes an extracellular epitope of CD74 (the MHC II-associated invariant chain, Ii), a type II transmembrane protein expressed in antigen-presenting cells, as well as e.g. on activated neoplastic cells in T cell lymphomas, in lymph node germinal center, mantle zone B cells, histiocytes, interdigitating reticulum cells, Langerhans cells, thymic dendritic cells and peripheral blood B lymphocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: SU-DHL-4 lymphoma cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: CD74 Molecule,CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment, it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.,p33, DHLAG, HLADG, Ia-gamma

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints