TCR alpha/beta antibody (PE)

Catalog No. ABIN1303123

This Mouse Monoclonal antibody specifically detects TCR alpha/beta in FACS. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for TCR alpha/beta antibody (PE) (ABIN1303123)

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR alpha/beta antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: IP26

Product Details anti-TCR alpha/beta Antibody (PE)

Purpose: Anti-Hu TCR alpha/beta PE

Specificity: The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-TCR alpha/beta antibody (PE) (ABIN1303123)

Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, (2009) (PubMed).

Ortonne, Huet, Gaudez, Marie-Cardine, Schiavon, Bagot, Musette, Bensussan: "Significance of circulating T-cell clones in Sezary syndrome." in: Blood, Vol. 107, Issue 10, pp. 4030-8, (2006) (PubMed).

Huet, Bagot, Loyaux, Capdevielle, Conraux, Ferrara, Bensussan, Marie-Cardine: "SC5 mAb represents a unique tool for the detection of extracellular vimentin as a specific marker of Sezary cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 1, pp. 652-9, (2005) (PubMed).

Target Details for TCR alpha/beta

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Alternative Name: TCR alpha/beta

Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.,TCRA/B