LY9 antibody (PE)

Catalog No. ABIN1303129

This Mouse Monoclonal antibody specifically detects LY9 in FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for LY9 antibody (PE) (ABIN1303129)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: HLy9-25

Product Details anti-LY9 Antibody (PE)

Purpose: Anti-Hu CD229 PE

Specificity: The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: CD299-transfected 300.19 pre-B cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-LY9 antibody (PE) (ABIN1303129)

Romero, Zapater, Calvo, Kalko, de la Fuente, Tovar, Ockeloen, Pizcueta, Engel: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).

de la Fuente, Tovar, Villamor, Zapater, Pizcueta, Campo, Bosch, Engel: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229

Background: Lymphocyte antigen 9,CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.,SLAMF3, LY9

Gene ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling