CASK antibody (AA 318-415)

Catalog No. ABIN1304569

This Mouse Monoclonal antibody specifically detects CASK in WB, IHC, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for CASK antibody (AA 318-415) (ABIN1304569)

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CASK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: K56A-50

Product Details anti-CASK Antibody

Binding Specificity: AA 318-415

Specificity: No off-targets reported

Cross-Reactivity: Chicken, Human, Mouse, Rat, Xenopus laevis, Zebrafish (Danio rerio)

Characteristics:

Description: Our Anti-CASK mouse monoclonal primary antibody is produced in-house from hybridoma clone K56A/50. It is KO validated, detects chicken, human, mouse, rat, Xenopus, and zebrafish CASK, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our CASK mouse monoclonal primary antibody from hybridoma clone K56A/50. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 318-415 of rat CASK/Lin2 (accession number Q62915) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: IHC: 1:500

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for CASK

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Alternative Name: CASK

Background:

Synonyms: Peripheral plasma membrane protein CASK (hCASK) (EC 2.7.11.1) (Calcium/calmodulin-dependent serine protein kinase) (Protein lin-2 homolog)

Target Description: Calcium/Calmodulin-Dependent Serine Protein Kinase or CASK scaffolding protein is encoded by the gene CASK. CASK is a member of the MAGUK (membrane-associated guanylate kinase) protein family. CASK is a a calcium/calmodulin-dependent serine protein kinase. CASK is found in many different tissues including kidney, lung, liver and brain. In the brain, CASK protein is located at synapses. CASK acts as a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Mutations in this gene are associated with FG syndrome 4, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability.

Gene Name Alternatives: CASK LIN2

Molecular Weight: 100 kDa

UniProt: Q62915

Pathways: Synaptic Vesicle Exocytosis