GIT2 antibody (AA 450-759)

Catalog No. ABIN1304675

The Mouse Monoclonal anti-GIT2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect GIT2 in samples from Human.

Quick Overview for GIT2 antibody (AA 450-759) (ABIN1304675)

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: N83-48

Product Details anti-GIT2 Antibody

Binding Specificity: AA 450-759

Specificity: No off-targets reported for GIT1

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-GIT2 mouse monoclonal primary antibody is produced in-house from hybridoma clone N83/48. It detects human, mouse, and rat GIT2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our GIT2 mouse monoclonal primary antibody from hybridoma clone N83/48. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Fusion protein amino acids 450-759 (C-terminus) of human GIT2 (accession number Q14161) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for GIT2

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Alternative Name: GIT2

Background:

Synonyms: ARF GTPase-activating protein GIT2 (ARF GAP GIT2) (Cool-interacting tyrosine-phosphorylated protein 2) (CAT-2) (CAT2) (G protein-coupled receptor kinase-interactor 2) (GRK-interacting protein 2)

Target Description: G-protein coupled receptor kinase interacting ArfGAP 2 is encoded by the gene GIT2. GIT2 is a member of the GIT protein family. GIT2 has a be shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. GIT2 is expressed in 207 organs with the highest expression level in the right hemisphere of cerebellum. Diseases associated with GIT2 include Gastric Antral Vascular Ectasia and Systemic Scleroderma.

Gene Name Alternatives: GIT2 KIAA0148

Molecular Weight: 85 kDa

UniProt: Q14161

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling