KCNA1 antibody (AA 191-208)

Catalog No. ABIN1304744

Product Details anti-KCNA1 Antibody

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Binding Specificity: AA 191-208

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: No off-targets reported

Cross-Reactivity: Mouse, Rat

Characteristics:

Description: Our Anti-Kv1.1 K+ channel (external) mouse monoclonal primary antibody is produced in-house from hybridoma clone K36/15. It is KO validated, detects mouse and rat Kv1.1 K+ channel (external), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Manufacturer Comment: We produce our Kv1.1 K+ channel (external) mouse monoclonal primary antibody from hybridoma clone K36/15. It is great in IHC, ICC, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Synthetic peptide amino acids 191-208 (ELKDDKDFTGTIHRIDNTC, extracellular domain) of rat Kv1.1 (accession number P10499)

Clone: K36-15

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

References for anti-KCNA1 antibody (ABIN1304744)

Chen, Koopmans, Gordon, Paliukhovich, Klaassen, van der Schors, Peles, Verhage, Smit, Li: "Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes." in: Biochimica et biophysica acta, Vol. 1854, Issue 7, pp. 827-33, (2015) (PubMed).

Zollinger, Chang, Baalman, Kim, Rasband: "The Polarity Protein Pals1 Regulates Radial Sorting of Axons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 29, pp. 10474-84, (2015) (PubMed).

Sosanya, Brager, Wolfe, Niere, Raab-Graham: "Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis." in: Neurobiology of disease, Vol. 73, pp. 96-105, (2014) (PubMed).

Jones, Corbin, Huntsman: "Neonatal NMDA receptor blockade disrupts spike timing and glutamatergic synapses in fast spiking interneurons in a NMDA receptor hypofunction model of schizophrenia." in: PLoS ONE, Vol. 9, Issue 10, pp. e109303, (2014) (PubMed).

Hoppa, Gouzer, Armbruster, Ryan: "Control and plasticity of the presynaptic action potential waveform at small CNS nerve terminals." in: Neuron, Vol. 84, Issue 4, pp. 778-89, (2014) (PubMed).

Kirizs, Kerti-Szigeti, Lorincz, Nusser: "Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells." in: The European journal of neuroscience, Vol. 39, Issue 11, pp. 1771-83, (2014) (PubMed).

Desmazieres, Zonta, Zhang, Wu, Sherman, Brophy: "Differential stability of PNS and CNS nodal complexes when neuronal neurofascin is lost." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 15, pp. 5083-8, (2014) (PubMed).

Dufour, Woodhouse, Goaillard: "Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development." in: Journal of neuroscience research, Vol. 92, Issue 8, pp. 981-99, (2014) (PubMed).

Campanac, Gasselin, Baude, Rama, Ankri, Debanne: "Enhanced intrinsic excitability in basket cells maintains excitatory-inhibitory balance in hippocampal circuits." in: Neuron, Vol. 77, Issue 4, pp. 712-22, (2013) (PubMed).

Lallet-Daher, Wiel, Gitenay, Navaratnam, Augert, Le Calvé, Verbeke, Carling, Aubert, Vindrieux, Bernard: "Potassium channel KCNA1 modulates oncogene-induced senescence and transformation." in: Cancer research, Vol. 73, Issue 16, pp. 5253-65, (2013) (PubMed).

Sosanya, Huang, Cacheaux, Chen, Nguyen, Perrone-Bizzozero, Raab-Graham: "Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1." in: The Journal of cell biology, Vol. 202, Issue 1, pp. 53-69, (2013) (PubMed).

Kyle, Ahrendt, Braun, Braun: "The large conductance, calcium-activated K+ (BK) channel is regulated by cysteine string protein." in: Scientific reports, Vol. 3, pp. 2447, (2013) (PubMed).

Liu, Lee, Davis: "Heterogeneous intrinsic excitability of murine spiral ganglion neurons is determined by Kv1 and HCN channels." in: Neuroscience, Vol. 257, pp. 96-110, (2013) (PubMed).

Bildl, Haupt, Müller, Biniossek, Thumfart, Hüber, Fakler, Schulte: "Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 2, pp. M111.007955, (2012) (PubMed).

Li, Lu, Xu, Zhang, Zhu, Zhang, Cao, Chen, Chen, Luo, Duan, Li: "Neuregulin 1 regulates excitability of fast-spiking neurons through Kv1.1 and acts in epilepsy." in: Nature neuroscience, Vol. 15, Issue 2, pp. 267-73, (2012) (PubMed).

Bangratz, Sarrazin, Devaux, Zambroni, Echaniz-Laguna, René, Boërio, Davoine, Fontaine, Feltri, Benoit, Nicole et al.: "A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is ..." in: The American journal of pathology, Vol. 180, Issue 5, pp. 2040-55, (2012) (PubMed).

Irani, Pettingill, Kleopa, Schiza, Waters, Mazia, Zuliani, Watanabe, Lang, Buckley, Vincent: "Morvan syndrome: clinical and serological observations in 29 cases." in: Annals of neurology, Vol. 72, Issue 2, pp. 241-55, (2012) (PubMed).

Zhu, Alsaber, Zhao, Ribeiro-Hurley, Thornhill: "Characterization of the Kv1.1 I262T and S342I mutations associated with episodic ataxia 1 with distinct phenotypes." in: Archives of biochemistry and biophysics, Vol. 524, Issue 2, pp. 99-105, (2012) (PubMed).

Menegola, Clark, Trimmer: "The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice." in: Epilepsia, Vol. 53 Suppl 1, pp. 142-9, (2012) (PubMed).

Giacomini, La Padula, Schenone, Leandri, Contestabile, Moruzzo, Goutebroze, Consalez, Benfenati, Corradi: "Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice." in: Neurobiology of disease, Vol. 42, Issue 1, pp. 73-84, (2011) (PubMed).

Target Details for KCNA1

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Alternative Name: Kv1.1 K+ channel (KCNA1 Products)

Background:

Synonyms: Potassium voltage-gated channel subfamily A member 1 (RBKI) (RCK1) (Voltage-gated potassium channel subunit Kv1.1)

Target Description: Potassium Channel Kv1.1or Kcna1 is a member of the Shaker potassium channel family. Kv1.1 is expressed in the brain, CNS and in the kidney. Potassium channels form homotetrameric and heterotetrameric channels in the membrane with various other related proteins, including KCNA2, KCNA4, KCNA5, KCNA6, KCNA7. Kv1.1 is involved with the regulation of cellular functions and the control of neuronal excitability. Mutations in Kv1.1 are associated with Episodic ataxia type 1 and epilepsy.

Gene Name Alternatives: Kcna1

Molecular Weight: 56 kDa (could be 65-85 kDa depending on glycosylation)

UniProt: P10499