SHANK3 antibody (AA 840-857)

Catalog No. ABIN1304956

This Mouse Monoclonal antibody specifically detects SHANK3 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for SHANK3 antibody (AA 840-857) (ABIN1304956)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: N69-46

Product Details anti-SHANK3 Antibody

Binding Specificity: AA 840-857

Specificity: No off-targets reported for Shank1 or Shank2

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-Shank3 mouse monoclonal primary antibody is produced in-house from hybridoma clone N69/46. It detects human, mouse, and rat Shank3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our Shank3 mouse monoclonal primary antibody from hybridoma clone N69/46. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Synthetic peptide amino acids 840-857 (PEKLPGSLRKGIPRTKSV) of rat Shank3 (accession number Q9JLU4)

Isotype: IgG2b

Application Details

Application Notes:

Dilution Range: IHC: 1:500

Dilution Range: ICC: 1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: Shank3

Background:

Synonyms: SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Target Description: SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Gene Name Alternatives: Shank3 Prosap2

Molecular Weight: 190 kDa

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity