Rat anti-Mouse IgA Antibody (PE)

Catalog No. ABIN135045

Quick Overview for Rat anti-Mouse IgA Antibody (PE) (ABIN135045)

Target: IgA

Clonality: Monoclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Clone: 11-44-2

Isotype: IgG1

Product Details

Reactivity: Mouse

Host: Rat

Specificity: Reacts with the heavy chain of mouse IgA

Characteristics: Rat Anti-Mouse IgA-PE

Purification: Protein A affinity column

Immunogen: Unknown

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , IHC-PS , WB
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:400 PE and APC conjugates 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use
Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Kubinak, Stephens, Soto, Petersen, Chiaro, Gogokhia, Bell, Ajami, Petrosino, Morrison, Potts, Jensen, OConnell, Round: "MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection." in: Nature communications, Vol. 6, pp. 8642, (2016) (PubMed).

Target Details

Target: IgA

Target Type: Antibody