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COL3 antibody (Biotin)

This anti-COL3 antibody is a Goat Polyclonal antibody detecting COL3 in ELISA. Suitable for Human. This Primary Antibody has been cited in 2+ publications.
Catalog No. ABIN135051

Quick Overview for COL3 antibody (Biotin) (ABIN135051)

Target

See all COL3 Antibodies
COL3 (Collagen, Type III (COL3))

Reactivity

  • 59
  • 42
  • 40
  • 18
  • 15
  • 1
  • 1
Human

Host

  • 51
  • 8
  • 7
Goat

Clonality

  • 55
  • 11
Polyclonal

Conjugate

  • 32
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This COL3 antibody is conjugated to Biotin

Application

  • 55
  • 28
  • 26
  • 26
  • 23
  • 16
  • 12
  • 11
  • 10
  • 7
  • 5
  • 2
  • 1
ELISA
  • Specificity

    Reacts with conformational determinants on type III collagen Referenced species reactivities include - Human 2,5,11,12,32,33,35-37 Chicken 22,23 Hamster 18,24 Mouse 4,6,13,15,34,39 Chinchilla 26 Porcine 1,5,17,29 Rat 5,10,14,20,31 Equine 25 Rabbit 9,16,19,21,38 Bovine 8,27 Feline 30 Rhesus 7 Canine 3,5 Guinea Pig 21 Tree Shrew 28

    Cross-Reactivity (Details)

    Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

    Characteristics

    Goat Anti-Type III Collagen-BIOT

    Purification

    Purification Method: Affinity chromatography on type III collagen covalently linked to agarose.

    Isotype

    IgG
  • Application Notes

    • Applications: Quality tested applications include - ELISA ,
    • Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , FC , WB
    • Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000

    Sample Volume

    0,5 mL

    Restrictions

    For Research Use only
  • Concentration

    0.4 mg/mL

    Buffer

    0.2 mg in 0.5 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Smeland, Lu, Karlsen, Salvesen, Reed, Stuhr: "Stromal integrin α11-deficiency reduces interstitial fluid pressure and perturbs collagen structure in triple-negative breast xenograft tumors." in: BMC cancer, Vol. 19, Issue 1, pp. 234, (2019) (PubMed).

    Buckley, Evans, Matuszewski, Chen, Satchel, Elliott, Soslowsky, Dodge: "Distributions of types I, II and III collagen by region in the human supraspinatus tendon." in: Connective tissue research, Vol. 54, Issue 6, pp. 374-9, (2014) (PubMed).

  • Target

    COL3 (Collagen, Type III (COL3))

    Alternative Name

    Type III Collagen
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