CD44 antibody (Biotin)

Catalog No. ABIN135067

This Rat Monoclonal antibody specifically detects CD44 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD44 antibody (Biotin) (ABIN135067)

Target: CD44

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: KM201

Product Details anti-CD44 Antibody (Biotin)

Specificity: Mouse CD44 (all isoforms), Mr 80-95 kDa

Characteristics: Rat Anti-Mouse CD44-BIOT

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

cell adhesion and homing

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling