CD3 epsilon antibody

Catalog No. ABIN135098

The Rat Monoclonal anti-CD3 epsilon antibody has been validated for FACS. It is suitable to detect CD3 epsilon in samples from Mouse.

Quick Overview for CD3 epsilon antibody (ABIN135098)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: C363-29B

Product Details anti-CD3 epsilon Antibody

Specificity: Mouse CD3, Mr 25 kDa

Characteristics: Rat Anti-Mouse CD3e-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: IL-4 producing Th2 cell lines including D10

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Activ - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, and CY5 conjugates 1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro depletion of CD3+ cells, In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway