CD19 antibody (FITC)

Catalog No. ABIN135154

This Mouse Monoclonal antibody specifically detects CD19 in FACS. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for CD19 antibody (FITC) (ABIN135154)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MB19-1

Product Details anti-CD19 Antibody (FITC)

Specificity: Mouse CD19, Mr 95 kDa

Characteristics: Mouse Anti-Mouse CD19-FITC

Immunogen: CD19+ mouse pre-B cell line 300.19

Isotype: IgA

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells APC/CY5.5 and APC/CY7 conjugates 0.3 g/106 cells PE, APC, SPRD, and PE/CY5.5 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD19 antibody (FITC) (ABIN135154)

Cumba Garcia, Huseby Kelcher, Malo, Johnson: "Superior isolation of antigen-specific brain infiltrating T cells using manual homogenization technique." in: Journal of immunological methods, Vol. 439, pp. 23-28, (2017) (PubMed).

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway