CD28 antibody

Catalog No. ABIN135185

The Hamster Monoclonal anti-CD28 antibody is suitable to detect CD28 in samples from Mouse. It has been validated for FACS.

Quick Overview for CD28 antibody (ABIN135185)

Target: CD28

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PV-1

Product Details anti-CD28 Antibody

Specificity: Mouse CD28, Mr 40 kDa

Characteristics: Hamster Anti-Mouse CD28-UNLB

Purification: Purified

Immunogen: C57BL/6N mouse T cell lymphoma EL4 cell line

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , Costim - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, and SPRD conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro costimulation of T cells and NK cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response