CD31 antibody

Catalog No. ABIN135196

This Rat Monoclonal antibody specifically detects CD31 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD31 antibody (ABIN135196)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 390

Product Details anti-CD31 Antibody

Specificity: Mouse CD31, Mr 130-140 kDa

Characteristics: Rat Anti-Mouse CD31-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PACBLU, and AF488 conjugates 1 g/106 cells PE, APC, PE/CY7 and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Blocking of cell adhesion

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and paltelets. CD31 interacts homotypically in cell adhesion assays. MAb 390 inhibits the aggregation of L cells transfected with a variant form of CD31.

Pathways: Regulation of Actin Filament Polymerization