CD32/CD16 antibody

Catalog No. ABIN135200

The Rat Monoclonal anti-CD32/CD16 antibody is suitable to detect CD32/CD16 in samples from Mouse. It has been validated for FACS, IP, BR and IHC (fro).

Quick Overview for CD32/CD16 antibody (ABIN135200)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 93

Product Details anti-CD32/CD16 Antibody

Purpose: Rat Anti-Mouse CD16/32-LE/AF

Specificity: Mouse CD16/32

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD16/32 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: VH81X Tg B cells and T220 stromal cell line

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/32

Background: The lymphocyte Fc receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying FcR+ cells, monoclonal antibodies to the FcII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcR for parasitic invasion.

Gene ID: 14131

UniProt: P08508