CD38 antibody

Catalog No. ABIN135206

The Rat Monoclonal anti-CD38 antibody has been validated for FACS. It is suitable to detect CD38 in samples from Mouse.

Quick Overview for CD38 antibody (ABIN135206)

Target: CD38

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD38 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 9E1

Product Details anti-CD38 Antibody

Specificity: Mouse CD38, Mr 42 kDa

Characteristics: Rat Anti-Mouse CD38-UNLB

Purification: Purified

Immunogen: Mouse bone marrow pre-B cells

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, and PACBLU conjugates 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Induction of B lymphocyte proliferation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD38

Target: CD38

Background: Murine CD38, a type II transmembrane glycoprotein, is a bifunctional ectoenzyme capable of catabolizing nicotinamide adenine dinucleotide (NAD+) to cyclic ADP-ribose (cADPR), and then hydrolyzing cADPR to adenosine diphosphoribose (ADPR). It is expressed at high levels on the surface of peripheral B-lineage cells, and at low density on germinal center B cells from unimmunized mice. It has also been reported to be expressed at moderate levels on NK cells, a proportion of peripheral T cells, and a subpopulation of thymocytes which are mostly TCR+, CD4-, CD8-. Murine CD38 is also expressed by all Mac-1+ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone-marrow-derived macrophages. Monoclonal antibodies to CD38 have been shown to induce B- and T-cell proliferation, protect B cells from apoptosis, and inhibit B lymphopoiesis.