ICAM1 antibody (Biotin)

Catalog No. ABIN135248

The Rat Monoclonal anti-ICAM1 antibody has been validated for FACS. It is suitable to detect ICAM1 in samples from Mouse.

Quick Overview for ICAM1 antibody (Biotin) (ABIN135248)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: YN1-1-7-4

Product Details anti-ICAM1 Antibody (Biotin)

Specificity: Mouse/Porcine CD54, Mr 95 kDa

Characteristics: Rat Anti-Mouse CD54-BIOT

Immunogen: Mouse NS-1 cells

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , IP - Reported in literature , Block - Reported in literature , Purification - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE, APC, AF647, and AF700 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro blocking studies

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN-γ, IL-1, TNF-α, lipopolysaccharide and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling