CD72.1 antibody (Biotin)

Catalog No. ABIN135266

The Mouse Monoclonal anti-CD72.1 antibody has been validated for FACS. It is suitable to detect CD72.1 in samples from Mouse.

Quick Overview for CD72.1 antibody (Biotin) (ABIN135266)

Target: CD72.1

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD72.1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 10-1-D2

Product Details anti-CD72.1 Antibody (Biotin)

Specificity: Mouse CD72.1, Mr 45 kDa

Characteristics: Mouse Anti-Mouse CD72.1-BIOT

Purification: Ion exchange

Immunogen: DBA/2 mouse spleen cells

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature , Activ - Reported in literature , Block - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells APC and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro blocking and proliferation assays

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD72.1

Target: CD72.1

Background: CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.