CD90.2 / Thy-1.2 antibody (PE)

Catalog No. ABIN135287

The Rat Monoclonal anti-CD90.2 / Thy-1.2 antibody has been validated for FACS. It is suitable to detect CD90.2 / Thy-1.2 in samples from Mouse. There are 3+ publications available.

Quick Overview for CD90.2 / Thy-1.2 antibody (PE) (ABIN135287)

Target: CD90.2 / Thy-1.2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90.2 / Thy-1.2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 30-H12

Product Details anti-CD90.2 / Thy-1.2 Antibody (PE)

Specificity: Mouse CD90.2 (Thy-1.2 alloantigen), Mr 25-30 kDa

Characteristics: Rat Anti-Mouse CD90.2-PE

Immunogen: Mouse thymus or spleen

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Costim - Reported in literature , Apop - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells APC, SPRD, PACBLU, and AF647 conjugates 0.2 g/106 cells AF700 conjugate 0.1 g/106 cells PE conjugate 0.05 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

in vivo and in vitro depletion of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD90.2 / Thy-1.2 antibody (PE) (ABIN135287)

Schubert, Sann, Frueh, Ullrich, Geiger, Baer: "Tracking of Adipose-Derived Mesenchymal Stromal/Stem Cells in a Model of Cisplatin-Induced Acute Kidney Injury: Comparison of Bioluminescence Imaging versus qRT-PCR." in: International journal of molecular sciences, Vol. 19, Issue 9, (2018) (PubMed).

Cooley, Martin, Zellner, Irani, Uram-Tuculescu, El Shikh, Conrad: "Increased B Cell ADAM10 in Allergic Patients and Th2 Prone Mice." in: PLoS ONE, Vol. 10, Issue 5, pp. e0124331, (2016) (PubMed).

Folgosa, Zellner, El Shikh, Conrad: "Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-? shedding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 12, pp. 5951-8, (2013) (PubMed).

Target Details for CD90.2 / Thy-1.2

Target: CD90.2 / Thy-1.2

Alternative Name: CD90.2

Background: CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues.