NK-1.1/CD161c antibody (FITC)

Catalog No. ABIN135341

The Mouse Monoclonal anti-NK-1.1/CD161c antibody has been validated for FACS. It is suitable to detect NK-1.1/CD161c in samples from Mouse.

Quick Overview for NK-1.1/CD161c antibody (FITC) (ABIN135341)

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: PK136

Product Details anti-NK-1.1/CD161c Antibody (FITC)

Specificity: Mouse NK1.1, Mr 39 kDa

Characteristics: Mouse Anti-Mouse NK1.1-FITC

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: CE mouse spleen enriched for NK-1+ cells and bone marrow cells

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, PACBLU, AF488, and AF700 conjugates 1 g/106 cells PE and APC conjugates 0.5 g/106 cells PE/CY7 and AF647 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Induction of proliferation of a subset of NK cells, In vivo depletion of NK1.1 cells, In vitro redirected lysis, In vitro cytotoxicity assays

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Alternative Name: NK1.1

Background: NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.