CD81 antibody (Biotin)

Catalog No. ABIN135368

The Hamster Monoclonal anti-CD81 antibody has been validated for FACS. It is suitable to detect CD81 in samples from Mouse.

Quick Overview for CD81 antibody (Biotin) (ABIN135368)

Target: CD81

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD81 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 2F7

Product Details anti-CD81 Antibody (Biotin)

Specificity: Mouse CD81, Mr 26 kDa

Characteristics: Hamster Anti-Mouse CD81-BIOT

Purification: Ion exchange column

Immunogen: Mouse epithelial cell line PAM212

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro blocking of thymocyte interactions with CD81

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background: CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of + T cells to more mature T cells with T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR chain and glycoprotein pT.

Pathways: Inositol Metabolic Process, Hepatitis C