LY6C + LY6G antibody (SPRD)

Catalog No. ABIN135407

The Rat Monoclonal anti- antibody has been validated for FACS. It is suitable to detect in samples from Mouse.

Quick Overview for LY6C + LY6G antibody (SPRD) (ABIN135407)

Target: LY6C + LY6G

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: SPRD

Application: Flow Cytometry (FACS)

Clone: RB6-8C5

Product Details

Brand: Spectral Red ™

Specificity: Mouse Ly-6G/Ly-6C, Mr 21-25 kDa

Characteristics: Rat Anti-Mouse Ly-6G/Ly-6C-SPRD

Immunogen: Unknown

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: LY6C + LY6G

Alternative Name: Ly-6G/Ly-6C

Background: Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G is expressed predominantly on neutrophils.