CD3 epsilon antibody (Biotin)

Catalog No. ABIN135466

This Mouse Monoclonal antibody specifically detects CD3 epsilon in FACS. It exhibits reactivity toward Pig.

Quick Overview for CD3 epsilon antibody (Biotin) (ABIN135466)

Target: CD3 epsilon (CD3E)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: PPT3

Product Details anti-CD3 epsilon Antibody (Biotin)

Specificity: Porcine CD3, Mr 23 kDa

Characteristics: Mouse Anti-Porcine CD3e-BIOT

Immunogen: Purified CD3 molecules from porcine thymus

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature , Stim - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway