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CD8 alpha antibody (FITC)

This anti-CD8 alpha antibody is a Mouse Monoclonal antibody detecting CD8 alpha in FACS. Suitable for Pig. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN135477

Quick Overview for CD8 alpha antibody (FITC) (ABIN135477)

Target

See all CD8 alpha (CD8A) Antibodies
CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity

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Pig

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD8 alpha antibody is conjugated to FITC

Application

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Flow Cytometry (FACS)

Clone

76-2-11
  • Specificity

    Porcine CD8α, Mr 35 kDa

    Characteristics

    Mouse Anti-Porcine CD8a-FITC

    Purification

    Ion exchange column

    Immunogen

    Fresh dd miniature swine thymocytes

    Isotype

    IgG2a
  • Application Notes

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Comment

    Cytotoxicity assays, In vivo depletion of CD8+ cells

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Ferrari, De Angelis, Martelli, Borghetti: "Swine virus-specific cytotoxic cells producing IFNgamma under different conditions of virus antigenic stimulation." in: Veterinary research communications, (2010) (PubMed).

  • Target

    CD8 alpha (CD8A) (CD8a Molecule (CD8A))

    Alternative Name

    CD8a

    Background

    Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or a CD8 heterodimer. It is found on most thymocytes, and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.

    Pathways

    TCR Signaling
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