CD8 alpha antibody (FITC)
Quick Overview for CD8 alpha antibody (FITC) (ABIN135477)
Target
See all CD8 alpha (CD8A) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Porcine CD8α, Mr 35 kDa
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Characteristics
- Mouse Anti-Porcine CD8a-FITC
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Purification
- Ion exchange column
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Immunogen
- Fresh dd miniature swine thymocytes
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Isotype
- IgG2a
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Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
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Comment
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Cytotoxicity assays, In vivo depletion of CD8+ cells
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Sample Volume
- 1 mL
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Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- 0.5 mg in 1.0 mL of PBS/Sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Storage
- 4 °C
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Storage Comment
- Store at 2-8°C
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Swine virus-specific cytotoxic cells producing IFNgamma under different conditions of virus antigenic stimulation." in: Veterinary research communications, (2010) (PubMed).
: "
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Swine virus-specific cytotoxic cells producing IFNgamma under different conditions of virus antigenic stimulation." in: Veterinary research communications, (2010) (PubMed).
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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Alternative Name
- CD8a
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Background
- Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or a CD8 heterodimer. It is found on most thymocytes, and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.
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Pathways
- TCR Signaling
Target
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