CD8 alpha antibody (PE)

Catalog No. ABIN135479

The Mouse Monoclonal anti-CD8 alpha antibody has been validated for FACS. It is suitable to detect CD8 alpha in samples from Pig. There are 2+ publications available.

Quick Overview for CD8 alpha antibody (PE) (ABIN135479)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 76-2-11

Product Details anti-CD8 alpha Antibody (PE)

Specificity: Porcine CD8α, Mr 35 kDa

Characteristics: Mouse Anti-Porcine CD8a-PE

Purification: Protein A

Immunogen: Fresh dd miniature swine thymocytes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Cytotoxicity assays, In vivo depletion of CD8+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (PE) (ABIN135479)

Guo, Fu, Wang, Lu, Wei, Tang, Fan, Liu, Zhang, Zhang, Huang, Liu, Li, Wu, Liu: "A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain." in: PLoS ONE, Vol. 7, Issue 7, pp. e41463, (2012) (PubMed).

Fort, Fernandes, Nofrarias, Díaz, Sibila, Pujols, Mateu, Segalés: "Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets." in: Veterinary immunology and immunopathology, Vol. 129, Issue 1-2, pp. 101-7, (2009) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 homodimer or a CD8 heterodimer. It is found on most thymocytes, and on the suppressor/cytotoxic subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.

Pathways: TCR Signaling