CD3 antibody (Biotin)

Catalog No. ABIN135506

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Chicken. There is 1 publication available.

Quick Overview for CD3 antibody (Biotin) (ABIN135506)

Target: CD3

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: CT-3

Product Details anti-CD3 Antibody (Biotin)

Specificity: Chicken/Pigeon CD3, Mr 20, 19 & 17 kDa

Characteristics: Mouse Anti-Chicken CD3-BIOT

Immunogen: Chicken thymocytes and Ig- blood mononuclear cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.3 g/106 cells SPRD conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro depletion of CD3+ cells, In vivo activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 antibody (Biotin) (ABIN135506)

Cheng, Zhu, Sun: "The immune enhancement of sodium lauryl sulfoacetate in chickens." in: Veterinary medicine international, Vol. 2010, pp. 485060, (2010) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3 is a member of the T cell receptor-associated CD3 complex.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway