CD8 alpha antibody (PE)

Catalog No. ABIN135515

The Mouse Monoclonal anti-CD8 alpha antibody has been validated for FACS. It is suitable to detect CD8 alpha in samples from Chicken. There are 4+ publications available.

Quick Overview for CD8 alpha antibody (PE) (ABIN135515)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CT-8

Product Details anti-CD8 alpha Antibody (PE)

Specificity: Chicken/Turkey/Guinea Fowl/Pigeon CD8α, Mr 34 kDa

Characteristics: Mouse Anti-Chicken CD8a-PE

Purification: Ion exchange column

Immunogen: Chicken thymocytes and Ig-negative blood leukocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and CY5 conjugates 0.2 g/106 cells APC, SPRD, AF647, and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (PE) (ABIN135515)

Han, Renu, Patil, Schrock, Feliciano-Ruiz, Selvaraj, Renukaradhya: "Immune Response to Salmonella Enteritidis Infection in Broilers Immunized Orally With Chitosan-Based Salmonella Subunit Nanoparticle Vaccine." in: Frontiers in immunology, Vol. 11, pp. 935, (2020) (PubMed).

Peng, Yu, Liang, Chi, Li, Jiang, Fang, Cui, Lai, Zhou, Zhou: "The mitochondrial and death receptor pathways involved in the thymocytes apoptosis induced by aflatoxin B1." in: Oncotarget, Vol. 7, Issue 11, pp. 12222-34, (2017) (PubMed).

Peng, Zhang, Bai, Ding, Zeng, Yang, Fang, Chen: "Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn." in: International journal of environmental research and public health, Vol. 11, Issue 8, pp. 8567-80, (2015) (PubMed).

Chen, Peng, Fang, Cui, Zuo, Deng, Chen, Geng, Lai, Tang, Yang: "Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1." in: International journal of environmental research and public health, Vol. 11, Issue 2, pp. 1904-13, (2015) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: CD8 In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80 % of thymocytes, 15 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow.

Pathways: TCR Signaling