CD3 antibody

Catalog No. ABIN135720

This anti-CD3 antibody is a Mouse Monoclonal antibody detecting CD3 in FACS. Suitable for Human.

Quick Overview for CD3 antibody (ABIN135720)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: UCHT1

Product Details anti-CD3 Antibody

Specificity: Human CD3, Mr 20 kDa

Characteristics: Mouse Anti-Human CD3-UNLB

Purification: Purified

Immunogen: Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Activ - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC, SPRD, CY5, PE/CY5.5, PE/CY7, 10 L/106 cells APC/CY7, PACBLU, AF488, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

in vitro activation of T Cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80 % of normal peripheral blood lymphocytes and on 10-20 % of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway