CD3 antibody (SPRD)

Catalog No. ABIN135724

This Mouse Monoclonal antibody specifically detects CD3 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD3 antibody (SPRD) (ABIN135724)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to SPRD

Application: Flow Cytometry (FACS)

Clone: UCHT1

Product Details anti-CD3 Antibody (SPRD)

Brand: Spectral Red ™

Specificity: Human CD3, Mr 20 kDa

Characteristics: Mouse Anti-Human CD3-SPRD

Immunogen: Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Activ - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC, SPRD, CY5, PE/CY5.5, PE/CY7, 10 L/106 cells APC/CY7, PACBLU, AF488, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

in vitro activation of T Cells

Restrictions: For Research Use only

Handling

Buffer: 100 tests in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80 % of normal peripheral blood lymphocytes and on 10-20 % of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway