CD28 antibody

Catalog No. ABIN1383845

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS, Func and IHC. It is suitable to detect CD28 in samples from Human. There are 5+ publications available.

Quick Overview for CD28 antibody (ABIN1383845)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (IHC)

Clone: B-T3

Product Details anti-CD28 Antibody

Specificity: Recognises the B7/BB1 receptor (B7/BB1 R), a 44 kDa protein

Purification: Ion exchange chromatography

Sterility: 0.22 μm filtered

Immunogen: U266 cell line,Myeloma X63/AG.8653 x Balb/c spleen cells

Isotype: IgG2a, kappa

Biological Activity Comment: Inhibits mixed lymphocyte culture. Co-stimulates T-cell proliferation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Stable at +2-8°C for 12 months. For longer storage freeze aliquots.

References for anti-CD28 antibody (ABIN1383845)

Vermot-Desroches, Sergent, Bonnin, Wijdenes: "Characterization of monoclonal antibodies directed against trail or trail receptors." in: Cellular immunology, Vol. 236, Issue 1-2, pp. 86-91, (2005) (PubMed).

Roth, Yssel, Pene, Chavez, Schertzer, Lansdorp, Spits, Luiten: "Telomerase levels control the lifespan of human T lymphocytes." in: Blood, Vol. 102, Issue 3, pp. 849-57, (2003) (PubMed).

Luiten, Péne, Yssel, Spits: "Ectopic hTERT expression extends the life span of human CD4+ helper and regulatory T-cell clones and confers resistance to oxidative stress-induced apoptosis." in: Blood, Vol. 101, Issue 11, pp. 4512-9, (2003) (PubMed).

Sauce, Bodinier, Garin, Petracca, Tonnelier, Duperrier, Melo, Apperley, Ferrand, Hervé, Lang, Tiberghien, Robinet: "Retrovirus-mediated gene transfer in primary T lymphocytes impairs their anti-Epstein-Barr virus potential through both culture-dependent and selection process-dependent mechanisms." in: Blood, Vol. 99, Issue 4, pp. 1165-73, (2002) (PubMed).

Jourdan, Abbal, Noraz, Hori, Uchiyama, Vendrell, Bousquet, Taylor, Pène, Yssel, Nora: "IL-4 induces functional cell-surface expression of CXCR4 on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 9, pp. 4153-7, (1998) (PubMed).

Target Details for CD28

Target: CD28

Alternative Name: CD28

Molecular Weight: 44 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response