PPT2 antibody (AA 221-302)

Catalog No. ABIN1385628

The Rabbit Polyclonal anti-PPT2 antibody (ABIN1385628) specifically detects PPT2 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for PPT2 antibody (AA 221-302) (ABIN1385628)

Target: PPT2 (Palmitoyl-Protein Thioesterase 2 (PPT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PPT2 Antibody

Binding Specificity: AA 221-302

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PPT2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPT2

Target: PPT2 (Palmitoyl-Protein Thioesterase 2 (PPT2))

Alternative Name: PPT2

Background:

Synonyms: Lysosomal thioesterase PPT2, Palmitoyl protein hydrolase 2, Palmitoyl protein thioesterase 2, PPT 2, PPT-2, Ppt2, PPT2_HUMAN, S thioesterase G14, S-thioesterase G14.

Background: PPT2 (palmitoyl-protein thioesterase 2), also known as G14, is a 302 amino acid glycosylated protein that localizes to the lysosome and belongs to the palmitoyl-protein thioesterase family. Expressed throughout the body with highest levels in skeletal muscle, PPT2 functions to remove thioester-linked fatty acyl groups from a variety of substrates, including S-palmitoyl-CoA, thereby playing an important role in lipid metabolism. PPT2 operates at an optimal pH of 7 and exhibits the highest activity for the acyl groups on myristic and palmitic acids, with lower levels of activity toward other short- and long-chain acyl substrates. PPT2 exists as two isoforms, one of which is expressed at low levels and is catalytically inactive.