CD73 antibody (AA 151-250)

Catalog No. ABIN1385637

This anti-CD73 antibody is a Rabbit Polyclonal antibody detecting CD73 in WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat, Cow, Dog and Sheep. This Primary Antibody has been cited in 13+ publications.

Quick Overview for CD73 antibody (AA 151-250) (ABIN1385637)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD73 Antibody

Binding Specificity: AA 151-250

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD73/NT5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD73 antibody (ABIN1385637)

Ma, Liu, Li, Lu, Bai, Fan, Guan, Guo: "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." in: Cryobiology, Vol. 75, pp. 7-14, (2017) (PubMed).

Long, Luo, Wang, Bates, Shetty: "Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells." in: Aging and disease, Vol. 8, Issue 3, pp. 301-313, (2017) (PubMed).

Lin, Fujita, Endo, Morita, Takeda, Nakagawa, Nishimura: "Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to Adipocytes in Canine Bone Marrow." in: Stem cells and development, Vol. 26, Issue 6, pp. 431-440, (2016) (PubMed).

Long, Li, Luo, Hei, Wang, Tian, Yang, Lei, Qiu, Liu: "MRI tracking of bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy." in: Neuroscience letters, Vol. 606, pp. 30-5, (2015) (PubMed).

Wang, Chen, Wu, Zhang, Hu, Ge, Yang, Xu, Liu, Ye: "Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD." in: International braz j urol : official journal of the Brazilian Society of Urology, Vol. 41, Issue 4, pp. 764-72, (2015) (PubMed).

Long, Hei, Luo, Tian, Yang, Li, Wei, Liu: "BMSCs transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." in: Neuroscience, Vol. 311, pp. 464-73, (2015) (PubMed).

Gao, Bai, Wang, Sun, Guan, Zheng: "All-trans retinoic acid promotes nerve cell differentiation of yolk sac-derived mesenchymal stem cells." in: Applied biochemistry and biotechnology, Vol. 174, Issue 2, pp. 682-92, (2014) (PubMed).

Lu, Xiong, Wang, Wang, Ma, Guan: "Isolation and characterization of adipose-derived mesenchymal stem cells (ADSCs) from cattle." in: Applied biochemistry and biotechnology, Vol. 174, Issue 2, pp. 719-28, (2014) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Gao, Bai, Xiong, Li, Shan, Huang, Ma, Guan: "Isolation and characterization of chicken dermis-derived mesenchymal stem/progenitor cells." in: BioMed research international, Vol. 2013, pp. 626258, (2013) (PubMed).

Long, Qiu, Wang, Yang, Jia, Xin, Wang, Han, Fei, Liu: "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." in: Brain research, Vol. 1532, pp. 1-13, (2013) (PubMed).

Wang, Long, Jia, Liu, Yi, Yang, Fei, Liu: "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells in vitro." in: Brain research bulletin, Vol. 99, pp. 84-94, (2013) (PubMed).

Takemitsu, Zhao, Yamamoto, Harada, Michishita, Arai: "Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells." in: BMC veterinary research, Vol. 8, Issue 1, pp. 150, (2012) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background:

Synonyms: NT, eN, NT5, NTE, eNT, CD73, E5NT, CALJA, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E

Background: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process